6/2/2023 0 Comments Barcode e1005 datasheet pdfLytic activity is lost in 5 minutes at 60☌ ![]() Β-1,3-glucan laminaripentao-hydrolase and β-1,3-glucanase (trace amount of protease, ca. Susceptible fungal genera: Asbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloekera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Saccharomyces, Saccharomyces, Saccharomycopsis, Schizosaccahromyces, and Torulopsis. R-Zymolyase includes 0.5 U/µl RNase A when reconstituted. ![]() Optimal Zymolyase activity is at 30° - 37☌ lytic activity ceases at higher temperatures. The main activities of the enzyme are β-1,3 glucanase and β-1,3-glucan laminaripentao-hydrolase, which hydrolyze glucose polymers at the β-1,3-glucan linkages releasing laminaripentaose as the principal product. The buffer has been optimized to confer maximal levels of enzymatic activity. The Zymolyase from Zymo Research is prepared from Arthrobacter luteus, lyophilized, and packaged with a resuspension buffer. ![]() The use of lytic enzymes like Zymolyase is routinely used for digestion. Digestion of yeast and fungal cell walls is necessary for many experimental procedures including spheroplasting, immunofluorescence, transformation, protein purification, and others.
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